HTRF IP standard curve. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Prepare your iD series instrument for shipment. Research Article Streptococcus lutetiensis Induces Autophagy via Oxidative Stress in Bovine Mammary Epithelial Cells Peng Chen ,1 Jingyue Yang,1 Naiwen Wu,1 Bo Han,1 John P. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. Open the catalog to page 1. SpectraMax i3x Multi-Mode Detection Platform User Guide. Figure 1. SpectraMax i3x Multi-Mode Detection Platform. com Check our website for a current listing of worldwide distributors. However, no piece of equipment is immune to breaking down. 635. com. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 3: The cuvette chamber. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. 800. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Bioz Stars score: 98/100, based on 1 PubMed citations. 1) Select the correct plate and the wells that you want to measure. ). Art-science interactive light show/animation. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. A mixture of 6-nitro-1H,3H-benzo[de]isochromene-1,3-dione (486 mg, 2. Simplified workflow: Add cellular imaging assays in a small footprint. Eliminate time-dependent substrate addition steps. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Examples: fiPress carefully to engage,fl fiDo not press down. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. We would like to show you a description here but the site won’t allow us. Example: [Drawer]. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Increased fluorescence performance. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Figure 3. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. . Find regional support. Find regional support. Multimode Readers Comparison Chart. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 304 signal level saturation. 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax i3x reader provides the. Microplate assays were performed on a multimode microplate detector (Molecular Devices Spectra Max i3x). SpectraMax i3x Multi-Mode Microplate Reader System. Amplitude : 1. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. Multimode Reader Comparison Chart. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an. Description 1. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. 1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 306 plate air cal. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. 10, and 0. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. Description 1. 7°C and the bottom to 36. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Expand your lab’s capabilities to include flash applications such as dual luciferase and. Singlet oxygen (1 O 2) generation was determined through 1,3-diphenylisobenzofuran (DPBF) quenching. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. applications, including dual luciferase and ATP assays. Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. Spectranet customer portalThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. Prepare your i3/i3x instrument for shipment. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 304 signal level saturation. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. 0´ 104 8. 2 x 10-14. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. Introduction. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Prepare your i3/i3x instrument for shipment. Description 1. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. The SpectraMax ® i3x reader provides the. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 2. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Introduction. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. 315 can't find zero order. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Detection of pro-inflammatory factors. 발광 (Luminescence) 리더기. Download PDF. Nuclear Receptor. For wavelengths in the UV range above 220 nm,. User-upgradeable detection modules allow you to expand the capabilities. comThe SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Prismatic colour and motion for everyone. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Explore a wealth of applications with the future-ready SpectraMax i3x. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The SoftMax Pro Software provides integrated instrument control. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. Table 1. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. It allows you to explore cellular pathways and protein activation and expression in one system. UV-Vis absorbance, fluorescence and luminescence • Accommodates plate types from six to 384-well format • Easily upgradeable with interchangeable filter cubesSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The SoftMax Pro Software quickly. 제품. See SpectraMax® Microplate Reader User Manual Download Page. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. applications, including dual luciferase and ATP assays. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. i3. 蛍光 発光 ELISA. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. Download PDF. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Settings not required by a reader are indicated by ‘---’. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. SpectraMax M 시리즈 Microplate Reader, Cuvette Port, IQ OQ 검증 | Molecular Devices. Molecular Devices Corporation. The plate was shaken and incubated in the dark for 10 minutes. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Cell counters for accurate and. SpectraMaxi3Multi. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. 12 mg/mL) of polysaccharides were prepared after addition of glucosum anhydricum for calibration. Find regional support. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Table 1. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. 305 reference level saturation. Italic and boldface type are used for emphasis. Note: Additional readers with similar performance are listed. , 2014; Xiong et al. We would like to show you a description here but the site won’t allow us. Features. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength and superior sensitivity across the full. Capture Flash Assays with Ease. Anne T. Carbonyl assay The plates for carbonyl detection were prepared as described. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. 315 can't find zero order. The SpectraMax ® i3x. Find regional support. 99 to $1999. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Molecular Devicesの全商品を見る. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Patented user-exchangeable cartridge design expands the system's detection capability making it. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 4. They were allowed to attach and grow overnight in a 37°C, 5% CO 2. Description 1. . Multi-Mode Reader. Table 1. 5. i3x. We would like to show you a description here but the site won’t allow us. SpectraMax i3x Multi-Mode Detection Platform. Multimode Reader Comparison Chart. 4. This instrument also provides the option of modular upgrades for Western Blotting,. ZERO BIAS - scores, article reviews, protocol conditions and more. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. Find regional support. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Meanwhile, the samples were detected with a fluorometric reader Spectra Max i3x (MD, America) at 488 nm excitation wavelength and 525 nm emission wavelength. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. Bioz Stars score: 98/100, based on 1 PubMed citations. Prime function 20 amol. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreSimilar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Align the two slots on the bottle holder with the two pegs on the rail. MTT was purchased from Sigma-Aldrich (St. The treatments were. The standard solutions (0. Prepare your i3/i3x instrument for shipment. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. It allows you to explore cellular pathways and protein activation and expression in one system. 6% for the on-board optics (monochromators). Native or oxidized AN (40 μM) and FN (0. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. 7. Home > Search Results > Danaher Inc > spectramax i3x. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Modularity does provide some measure of forward vision into upcoming or future experimental needs. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Synthesis of ZM-1NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Microplate Reader. Supplier: Molecular Devices. ) deep, preferably with access on all sides. It allows you to explore cellular pathways and protein activation and expression in one system. 3) At the bottom of the strip, click on "Show. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The calculated LOQ values were 0. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Find regional support. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. ) wide and 65 cm (25. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the. Assay setup for a 384-well low-volume plate. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. 8 Antibodies and. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Figure 2-23: Bottle holder To install the bottle holder: 1. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. Sunnyvale, CA, USA) with excitation at 475 nm. Published Dec 16, 2020 | Updated Dec 16, 2020. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. Manufacturers try to design their instruments so they can be. We would like to show you a description here but the site won’t allow us. Then, the cell area was calculated for each. The SpectraMax i3 is specifically used in this lab for BCA assays and Elisa Assays. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. 2. iD5. g. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the datathe SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge Introduction Reporter gene assays are used to study the expression of eukaryotic genes. Ease of use. Wokingham, Berkshire RG41 5TS United Kingdom. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Also refer to this Multimode Reader Comparison Chart. Check software compatibility with Windows 10 and locate your software installer. 1. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. techsupport. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. +44 118 944 8000. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . 1. 660-665 Eskdale Road. Shake intensity. Detection cartridge modularity allows for easy addition of new read modes within minutes without. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Figure 4. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. Generate more data points without the need to dilute. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 1step assay with SpectraMax microplate readers. Microplate Stacker. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Microplate reader assays requiring infrared (IR) or red-shifted. Spectranet customer portal The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Prepare your iD series instrument for shipment. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. #0200-7009) The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Prime function 20 amol. Each heating element is independently. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Detection of pro-inflammatory factors. Description 1. The plate was covered and incubated for one hour at room temperature. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Methods. MD Neurotransmitter Transporter Assay. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. 0. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. pathways and protein activation and expression in one system. The dual auto injector module. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Winnersh Triangle. Spectranet customer portalSpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer by Molecular Devices® Manufacturer Molecular Devices® | Model: i3x | Available Worldwide 4. , 1997). SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Time-. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . When the user applies a set temperature of 37°C, the top is heated to 37. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 2. It allows you to explore cellular pathways and protein activation and expression in one system. Figure 4. 2 (Molecular Devices, San Jose, CA, USA). 1. Fluorescent Polarization: Solid black plates, as above. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. The fluorescence spectra were acquired using a microplate reader (Spectra Max i3x; Molecular Devices, USA). Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). spectramax i3x (Danaher Inc)The fluorescence emission intensity spectra were recorded using a microplate reader (Spectra Max® i3x, Molecular Devices, LLC. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 0´ 104 1. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. ; The top 3 importers of Spectramax are India with 365. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). , Fisher Scienti fi c, cat. Hands-on fun and learning that empowers exploring, inventing and sharing. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 0´ 104 6. QBT Fatty Acid Transporter Assay. These robust readers have been placed in labs from Antarctica to the. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. 2. Description 1. Increased fluorescence performance. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. The chamber. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Other services inlcude but are not limited to anti-drug activity (ADA), PK/PD assays as well as antibody screenings. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The ratio of the two fluorescence intensities 520/620 (acceptor/donor) enables the calculation of Delta F (%) which. A: percent GFP-positive cells calculated using the classification feature in the software. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. Example: [Drawer]. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Download PDF. 2 in. It features a modular design that allows users to add or change detection cartridges according to their needs. Learn more about its features, specifications, and applications in this user guide.